Novelβ-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase fromAcremoniumsp. HI-25
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چکیده
منابع مشابه
N-Linked High Mannose-type Oligosaccharides
Incubation of Crithidia fasciculata cells with [U-’“C] glucose led to the synthesis of Man-P-dolichol but not of Glc-P-dolichol. The main and largest dolicho1-P-Plinked oligosaccharide formed was Man7GlcNAcz whether labeling was performed in 5 mM sodium pyruvate or 5.5 mM glucose. The protein-linked, endo-j3-N-acetylglucosaminidase H-sensitive oligosaccharides isolated from mature glycoprote...
متن کاملStructural studies of phosphorylated high mannose-type oligosaccharides.
Phosphomannosyl residues on acid hydrolases serve as recognition markers which target these enzymes to lysosomes. We have found that the oligosaccharide units of newly synthesized beta-glucuronidase contain phosphate residues in diester linkage between mannose and alpha-linked N-acetylglucosamine residues (Tabas, I., and Kornfeld, S. (1980) J. Biol. Chem. 255, 6633--6639). To obtain larger amou...
متن کاملSynthesis of galactofuranose-containing disaccharides using thioimidoyl-type donors.
Four galactofuranose-containing disaccharides have been prepared utilising various thioimidates [Galf-SC(NR)XR'] and suitably protected acceptors as key precursors. We observed that the efficiency of the coupling reactions was particularly dependent on the aglycon present on the furanosyl donor when copper(II) ions were used as the promoter, and that activation could be correlated with the natu...
متن کاملAscorbate oxidase.
A new procedure has been described for the isolation and purification of ascorbate oxidase from green zucchini squash (Cucurbita pepo meduttosa). The new purification method achieves a more than ZOO-fold purification with about a 14% recovery of the total activity present in the crude juice. The purified enzyme preparations have been found to be homogeneous by the criteria of ultracentrifugal a...
متن کاملHigh-mannose dolichol-linked oligosaccharides in infantile ceroid-lipofuscinosis.
acid. Tryptic peptides were prepared from the oligosaccharide-containing CNBr peptide (see below) by incubation at 2 mg/ml in 2% (w/v) ammonium bicarbonate, pH 8.0, with trypsin (Worthington TRTPCK; E:S of I : 100) at 25°C for 3 h, followed by freeze-drying. The peptides were dissolved in 0.1% (v/v) trifluoracetic acid and separated on a 4.6 mmx250 mm C-18 reverse-phase column (Vydac 201TP 104;...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1996
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.60.1123